It's already been established that she's got the hots for him... If I can get the archive to load, I'll hunt up the comic.
When she first joined Team Evil, she explained that she loved the undead. Yes, she made it quite clear, she meant in that sense. Xykon, however, finds relations with the living disgusting and perverted. (I'm paraphrasing.)
So it makes sense that Tsukiko would have a doll to sleep with that reflected her unrequited fantasies.
Zardoz, there's TONS of places where V has been called "she". There's also tons of places where V has been called "he".
And yet every single time some new page does it again, there's always some person acting as if this happened for the first time, as if there's some revelation that occured.
It didn't. V remains gender-ambiguous.
Indeed, Rich has often said in the forums on his own site that any in-character reference to Vaarsuvius' sex is just an expression of that character's own belief, and the characters don't know any better than we do.