Generally not an issue with my gang. However, it's becoming a running joke with my current group that somehow, no one can remember my wife is a male dwarf with a 6 Charisma. So other than,
"What is she doing?"
"He!"
" ...he doing?"
Funny anecodate time. It involves one of my exes, who LARPs, role-plays by e-mail, and goes to ren fairs, but does not play RPGs. So I was in a 3rd edition campaign, and we were playing at a friend's house, so my then-girlfriend came along to kibitz (I offered many times she could stay home or arrange to hang out with someone else, but she always insisted she wanted to come along, sooo....).
We were driving home after a game.
She: It's so funny.
Me: What is?
She: You can always tell when a guy is trying to play a girl.
Me: I'm not sure what you mean by that.
She: Like, the prissy voice and stuff. It's hilarious. It sounds nothing like a woman.
Me: So... an example?
She: You.
Me: Me?
She: Uh, yeah. You.
Me: What do you mean?
She: The way you were playing.
Me: What's wrong with the way I was playing?
She: Just what I said.
Me: You mean, about playing female characters?
She: Yeah.
Me: My character is male.
She: ... Oh.
Me: Hahahahaha!
She: [rolls eyes]